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Stock IDSD01812
Strain NameBMCG-B2086
AKA
SpeciesT. thermophila
Genetic Descriptionin B2086 (MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; Cna1p-GFPc, green centromeres, II)
MAC Knockins/outsGFP tagged CNA1 from the MTT1 locus
MIC Knockins/outs
Gene of InterestCNA1 (TTHERM_00146340), CenH3, CENP-A
OriginBowen Cui
PublicationsCui, B. and Gorovsky, M.A. 2006. Centromeric Histone H3 is essential for vegetative cell division and for DNA elimination during conjugation in Tetrahymena. Mol. Cell Biol. 26: 4499-4510.
CommentsCentromeric H3 protein, green by IF. Partial mac replacement of MTT1 gene with GFP c-terminal tagged CNA1 gene. Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01812
Requires MTA?No
Academic cost $35