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Stock IDSD01811
Strain NameBMCG- ∆TWI11-5
SpeciesT. thermophila
Genetic Descriptiontwi11[∆?::neo3]/twi111[∆?::neo3] (twi11[∆?::neo3]/twi111[∆?::neo3]; MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; bsr-r, pm-r, GFP tagged Cna1p, ?)
MAC Knockins/outsCNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
MIC Knockins/outsTWI11 replaced by neo3
Gene of InterestCNA1 (TTHERM_00146340), TWI11 (TTHERM_00144830)
OriginBowen Cui
PublicationsCui B and Gorovsky MA. 2006. Centromeric histone H3 is essential for vegetative cell division and for DNA elimination during conjugation in tetrahymena thermophila. Mol Cell Biol 26(12):4499-510.

For info on any TWI11 contact Tomoko Noto
Comments∆TWI11 homokaryon transformed with Bsr-MTT1-CNA1-GFP.

CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cassette was inserted at the 5’F seq. of MTT1 locus. The construct was transformed into mac.

Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01811
Requires MTA?No
Academic cost $35