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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01783
Strain NameVI012
SpeciesT. thermophila
Genetic DescriptionMPR1/mpr1-1; PAR1/par1-1[Δ17-2587::neo2] (MPR1/mpr1-1; PAR1/par1-1[Δ17-2587::neo2]; mp-r, pm-r, ?)
MAC Knockins/outs
MIC Knockins/outsGCP3 gene partially replaced by neo4-KanR2 cassette.
Gene of InterestPAR1 (TTHERM_00502600)
OriginGermline biolistic transformation of mating B2086 and CU428 strains using a neo2 knockout cassette.
CommentsThe knockout has been confirmed by 6-methylpurine and paromomycin resistance, but neither micronuclear nor macronuclear allele homozygosity has been verified. The neo2 cassette has been inserted in the reverse orientation.

Submitted by Robert Coyne, J. Craig Venter Institute
Research Resource IdentifierTSC_SD01783
Requires MTA?No
Academic cost $45