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We have resumed shipping live cells and will soon ship plasmids and extracted DNA. Transformation services will take longer to resume.

Stock IDSD01779
Strain NameS-H3.3+GFP/H3.3-79
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,GFPc]; pm-r, VII)
MAC Knockins/outsC-terminal GFP- tag of HHT3, not sure if complete replacement
MIC Knockins/outs
Gene of InterestHHT3 (TTHERM_00016170)
OriginBowen Cui
PublicationsCui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsSomatic replace HHT3 gene with HHT3-GFP sequence. Neo2 in 5’ flanking of HHT3.

Mac transformant,GFP tagged HHT3, green nuclei.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD01779
Requires MTA?No
Academic cost $45