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We have resumed shipping live cells and will soon ship plasmids and extracted DNA. Transformation services will take longer to resume.

Stock IDSD01775
Strain NameVI010
SpeciesT. thermophila
Genetic DescriptionMPR1/mpr1-1; JMJ2/jmj2-1[Δ21-672::neo2] (MPR1/mpr1-1; JMJ2/jmj2-1[Δ21-672::neo2]; mp-r, pm-r, ?)
MAC Knockins/outs
MIC Knockins/outsJMJ2 gene partially replaced by neo4-KanR2 cassette.
Gene of InterestJMJ2 (TTHERM_00455500)
OriginGermline biolistic transformation of mating B2086 and CU428 strains using a neo2 knockout cassette.
CommentsThe knockout has been confirmed by 6-methylpurine and paromomycin resistance, but neither micronuclear nor macronuclear allele homozygosity has been verified.

Submitted by Robert Coyne, J. Craig Venter Institute
Research Resource IdentifierTSC_SD01775
Requires MTA?No
Academic cost $45