Tetrahymena Stock Center| Stock ID | SD01738 |
| Strain Name | VI001 |
| AKA | |
| Species | T. thermophila |
| Genetic Description | MPR1/mpr1-1; PAR1/par1-1[Δ17-2587(::neo4, KanR2)] (MPR1/mpr1-1; PAR1/par1-1[Δ17-2587(::neo4, KanR2)]; mp-r, pm-r, ?) |
| MAC Knockins/outs | |
| MIC Knockins/outs | PAR1 gene partially replaced by neo4-KanR2 cassette. |
| Gene of Interest | PAR1 (TTHERM_00502600) |
| Origin | Germline biolistic transformation of mating B2086 and CU428 strains using a neo4-KanR2 knockout cassette. |
| Publications | |
| Comments | The knockout also introduces a frameshift mutation. The knockout has been confirmed by 6-methylpurine and paromomycin resistance, but neither micronuclear nor macronuclear allele homozygosity has been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena. Submitted by Robert Coyne, J. Craig Venter Institute |
| Research Resource Identifier | TSC_SD01738 |
| Requires MTA? | No |
| Academic cost | $45 |
