Stock ID | SD01628 |
Strain Name | BI3840 IVa |
AKA | pig |
Species | T. thermophila |
Genetic Description | Not applicable (pig?; chx1-1; pig, Cy-R, IV) |
MAC Knockins/outs | |
MIC Knockins/outs | |
Gene of Interest | |
Origin | Originally isolated as a pigment-excreting mutant, "pig" (see Kaney and Knox, 1980); later discovered to be amicronucleate (Kaney and Speare, 1983). Stock culture obtained from A. Kaney in Feb. 1982. |
Publications | Kaney AR and Knox GW. 1980. Production of melanin precursors by a mutant of tetrahymena thermophila. J Protozool 27(3):339-41. Kaney AR and Speare VJ. 1983. An amicronucleate mutant of tetrahymena thermophila. Exp Cell Res 143(2):461-7. Karrer K, Stein-Gavens S, Allitto BA. 1984. Micronucleus-specific DNA sequences in an amicronucleate mutant of tetrahymena. Dev Biol 105(1):121-9. Kaney AR. 1985. A transmissible developmental block in tetrahymena thermophila. Exp Cell Res 157(2):315-21. Frankel J and Nelsen EM. 1986. Intracellular pattern reversal in tetrahymena thermophila. II. transient expression of a janus phenocopy in balanced doublets. Dev Biol 114(1):72-86. |
Comments | BI3840 cannot indefinitely maintain an introduced micronucleus (Kaney and Speare, 1983) and is developmentally defective (Kaney, 1985). Some DNA sequences that are limited to the micronucleus in wild-type cells are present in the macronucleus in BI3840 (Karrer et al., 1984). BI3840 has a tendency to produce homopolar doublets (Frankel & Nelsen, 1986). This stock probably corresponds to clone #1 in Frankel & Nelsen, (1986), isolated on 3/1/82. Submitted by Joseph Frankel, University of Iowa |
Research Resource Identifier | TSC_SD01628 |
Requires MTA? | No |
Academic cost | $45 |