Skip to main content

Follow @TetrahymenaSC

We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01596
Strain NameMU125
SpeciesT. thermophila
Genetic Descriptionasi2::neo/asi2::neo, MPR1/MPR1, CHX1/CHX1 (ASI2, chx1-1; pm-s, cy-r, IV)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestASI2 (TTHERM_00191480)
OriginMU119 was crossed with CU427 and progeny were allowed to assort for paromomycin sensitivity and cycloheximide resistance. This was clone 8C-8A. MU125 was the product of a Round I genomic exclusion mating between 8C-8A and B*7. The 8C-8A side was selected on the basis of cycloheximide resistance. Clones were crossed with CU428 to determine the micronuclear genotype for the ASI2 knockout allele and the CHX1 gene.
CommentsCrosses were done by Susan T. Gater.

Submitted by Kathleen Karrer, Marquette University
Research Resource IdentifierTSC_SD01596
Requires MTA?No
Academic cost $45