| Stock ID | SD01503 |
| Strain Name | SB1457 |
| AKA | tyrD6 |
| Species | T. thermophila |
| Genetic Description | gal1-1/gal1-1; tyrD6/tyrD6 (gal1-1; tyrD6; gal-r; tyr auxotroph, VII) |
| MAC Knockins/outs | |
| MIC Knockins/outs | |
| Gene of Interest | |
| Origin | SB210 cells were exposed to nitrosoguanidine and crossed to CU329 cells. Self-fertilization (cytogamy) was induced. Self-fertilized progeny were selected for by exploiting the recessiveness of the 2-deoxygalactose-resistance mutation. The cloned progeny were then replicated to rich medium and defined medium at 38°C, and those that grew poorly or not at all in defined medium but well in complex medium, were then tested in defined medium + tyrosine. |
| Publications | Sanford YM and Orias E. 1981. Phenylketonuric tetrahymena: Phenylalanine hydroxylase mutants and other tyrosine auxotrophs. Proc Natl Acad Sci U S A 78(12):7614-8. |
| Comments | Submitted by Eduardo Orias, University of California, Santa Barbara |
| Research Resource Identifier | TSC_SD01503 |
| Requires MTA? | No |
| Academic cost | $45 |
Tetrahymena Stock Center