Stock ID | SD01458 |
Strain Name | IA225 |
AKA | cdaE-3 IIA |
Species | T. thermophila |
Genetic Description | cdaE-3/cdaE-3 (cdaE-3; II) |
MAC Knockins/outs | |
MIC Knockins/outs | |
Gene of Interest | CDAE (cell division arrest E) |
Origin | Genomic exclusion progeny following an outcross to B2079-X-VI of a mutant clone that resulted from nitrosoguanidine mutagenesis in 1979 of SB210 followed by cytogamy. |
Publications | None. The allelic cdaE-1/cdaE-1 (IA137) was described in: Frankel J, Jenkins LM, Doerder FP, Nelsen EM. 1976. Mutations affecting cell division in tetrahymena pyriformis. I. selection and genetic analysis. Genetics 83(3):489-506. Frankel J, Nelsen EM, Jenkins LM. 1977. Mutations affecting cell division in tetrahymena pyriformis, syngen 1. II. phenotypes of single and double homozygotes. Dev Biol 58(2):255-75. |
Comments | Incomplete fission-zone formation with arrest in late-cytokinesis, with the new oral apparatus often abnormal and frequently wedged within the arrested furrow. Expressed in many cells at normal growth temperatures, near 100% at 39°. Same phenotype as the allelic IA137 stock,but with stronger expression. Submitted by Joseph Frankel, University of Iowa |
Research Resource Identifier | TSC_SD01458 |
Requires MTA? | No |
Academic cost | $45 |