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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01420
Strain NameVB1
SpeciesT. thermophila
Genetic Description(btu1[K350M]; ory-r, vbl--r)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestBTU1 (TTHERM_00348510)
OriginLong Gu
PublicationsGaertig J, Thatcher TH, Gu L, Gorovsky MA. 1994. Electroporation-mediated replacement of a positively and negatively selectable beta-tubulin gene in tetrahymena thermophila. Proc Natl Acad Sci U S A 91(10):4549-53.

Gu L, Gaertig J, Stargell LA, Gorovsky MA. 1995. Gene-specific signal transduction between microtubules and tubulin genes in tetrahymena thermophila. Mol Cell Biol 15(9):5173-9.
CommentsCU428 x B2086 mac transformant with BTU1 LYS(350) replaced by MET(350)

See also CU522 as another taxol sensitve strain for transformation.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01420
Requires MTA?No
Academic cost $45