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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01417
Strain NameTTMGG A1
SpeciesT. thermophila
Genetic Descriptiongtu1[∆::neo2]/gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆::GTU1,GFPc]; pm-r, ?)
MAC Knockins/outsGTU1with c-terminal GFP tag into MTT1 locus, partial replacement
MIC Knockins/outsGTU1 replaced by neo2
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.

Shang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44.
CommentsIn mac at GTU locus, GTU coding region is replaced by neo cassette. At metallothionein locus, two alleles a) MTT coding region, b) GTU coding region with GFP tag . Use the pTTMGG construct (GTU coding, GFP tag driven by MTT promoter) to rescue GTU KO heterokaryon.

Requires Cd to grow, Use .25 to 1 ug/ml. GFP not detected at protein level.
Research Resource IdentifierTSC_SD01417
Requires MTA?No
Academic cost $45