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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD01373
Strain NameH3.2-Flag-6
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (hht2[3’ neo2,FLAGc]; pm-r, VII)
MAC Knockins/outshht2[3’ neo2,FLAGc]
MIC Knockins/outs
Gene of InterestHHT2 (TTHERM_00189180)
OriginBowen Cui
PublicationsYu L and Gorovsky MA. 1997. Constitutive expression, not a particular primary sequence, is the important feature of the H3 replacement variant hv2 in tetrahymena thermophila. Mol Cell Biol 17(11):6303-10.

Cui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsSomatically replace HHT2 gene with HHT2-Flag sequence, neo 2 cassette is inserted in the 3’ flanking region of HHT2. Unknown if replacement is complete.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01373
Requires MTA?No
Academic cost $45