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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD01362
Strain Namegl∆H3/H4 major, #7
AKA
SpeciesT. thermophila
Genetic Descriptionhhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (mp-r, VII)
MAC Knockins/outs
MIC Knockins/outsNeo KO of all major HHT genes and HHF genes
Gene of InterestHHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
OriginYifan Liu
PublicationsLiu Y. 2003. Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of tetrahymena thermophila. University of Rochester.

Liu Y, Song X, Gorovsky MA, Karrer KM. 2005. Elimination of foreign DNA during somatic differentiation in tetrahymena thermophila shows position effect and is dosage dependent. Eukaryot Cell 4(2):421-31.
CommentsGermline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It mates with gl∆H3/H4 #4 strain.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01362
Requires MTA?No
Academic cost $45