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Stock IDSD01341
Strain NamecTTMG-HA #14
AKA
SpeciesT. thermophila
Genetic Descriptiongtu1[∆::neo2]/gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆::GTU1,HAc]; pm-r, ?)
MAC Knockins/outsGTU1with c-terminal HA tag into MTT1 locus, partial replacement
MIC Knockins/outsGTU1 replaced by neo2
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.

Shang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44.
CommentsIn mac at GTU locus, GTU coding region is replaced by neo cassette. At metallothionein locus, two alleles a) MTT coding region, b) GTU coding region with HA tag .Progeny of GTUKO5x6, transformed with pTTMGHA construct (GTU coding, HA tag driven by MTT promoter) to rescue GTU KO heterokaryon. Can mate with cTTMG-HA #12 or #15.

Requires Cd to grow, use .25 to 1 ug/ml. GTU1-HA detected at protein level.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01341
Requires MTA?No
Academic cost $35