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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD01216
Strain NamecTTMGHA
AKAcTTMG-HA #12
SpeciesT. thermophila
Genetic Descriptiongtu1::neo2/gtu1::neo2;Chx1/Chx1; Mpr?/Mpr? (MTT1/GTU1[HA]; gtu1::neo2/gtu1::neo2; pm-r, BTU1 has(E431,435D), BTU2 is replaced by bsr1, ?)
MAC Knockins/outsHA tagged GTU1 gene into the MTT1 coding region
MIC Knockins/outsGTU1 gene replaced by neo2 cassette
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.
CommentsProgeny of GTUKO5 x GTUKO6 mating, rescued by biolistic transformation with pTTMG-HA ( GTU-HA fusion driven by MTT1 promoter at the MTT1 locus). Needs 0.1μg/ml to 0.5 μg/ml Cd to grow, depending on the growth medium. Can cross with cTTMG-HA#15 or #14

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD01216
Requires MTA?No
Academic cost $45