Stock ID | SD01215 |
Strain Name | tG2NHA-1 |
AKA | 23-2 |
Species | T. thermophila |
Genetic Description | mpr1-1/mpr1-1 (MPR1/MPR1, gtu1[cHA]::neo2/gtu1[cHA]::neo2; pm-r, VII) |
MAC Knockins/outs | GTU1 gene completely replaced by GTU1 gene with HA tag at the C terminus. Also has neo2 cassette in the 3' flanking region |
MIC Knockins/outs | |
Gene of Interest | GTU1 (TTHERM_00079520) |
Origin | Yuhua Shang |
Publications | Shang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206. |
Comments | Somatic biolistic transformation of CU428 cells with gamma tubulin GTU1 gene with HA tag at C-terminus and neo2 cassette in the 3' flanking region of the gene. Complete replacement in mac. From the Gorovsky lab, University of Rochester. |
Research Resource Identifier | TSC_SD01215 |
Requires MTA? | No |
Academic cost | $45 |