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Stock IDSD01215
Strain NametG2NHA-1
AKA23-2
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (MPR1/MPR1, gtu1[cHA]::neo2/gtu1[cHA]::neo2; pm-r, VII)
MAC Knockins/outsGTU1 gene completely replaced by GTU1 gene with HA tag at the C terminus. Also has neo2 cassette in the 3' flanking region
MIC Knockins/outs
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.
CommentsSomatic biolistic transformation of CU428 cells with gamma tubulin GTU1 gene with HA tag at C-terminus and neo2 cassette in the 3' flanking region of the gene. Complete replacement in mac.

From the Gorovsky lab, University of Rochester.
Research Resource IdentifierTSC_SD01215
Requires MTA?No
Academic cost $35