Stock ID | SD00635 |
Strain Name | IA342 |
AKA | bcd1-1 II |
Species | T. thermophila |
Genetic Description | bcd1-1/bcd1-1; eja1-1/eja1-1 (bcd1-1; eja1-1; bcd1, eja1, II) |
MAC Knockins/outs | |
MIC Knockins/outs | |
Gene of Interest | BCD1 (broadened cortical domains); EJA1 (enhancer of janus-A) |
Origin | Genomic exclusion progeny following two successive generations of outcrossing to IA264 of a mutant clone resulting from nitrosoguanidine mutagenesis of IA264 in 1984 followed by cytogamy. Sister clone to IA344. |
Publications | Gene and this clone: Cole ES, Frankel J, Jenkins LM. 1987. Bcd: A mutation affecting the width of organelle domains in the cortex of tetrahymena thermophila. Development Genes and Evolution 196(7):421-33. Cole ES, Frankel J, Jenkins LM. 1988. Interactions between janus and bcd cortical pattern mutants in tetrahymena thermophila. Development Genes and Evolution 197(8):476-89. Cole ES and Stuart KR. 1991. Biochemical and cytological evidence for an overabundance of mucocysts in the bcd pattern mutant of tetrahymena thermophila. J Protozool 38(6):537-47. |
Comments | A mutation that brings about a broadening of oral and CVP domains, originally thought to be non-temperature sensitive, but capable of expressing an extreme phenotype at high temperature (Cole et al. 1988). Exhibits conjugal block. There is no evidence for any effect of eja1-1 on expression of bcd. |
Research Resource Identifier | TSC_SD00635 |
Requires MTA? | No |
Academic cost | $45 |