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Plasmid IDPID00036
Plasmid NameIIA8
Academic Cost$50
Base Pairs6300
SourceKathleen Karrer, Marquette University, U.S.A.
PublicationsKarrer KM. 1983. Germ line-specific DNA sequences are present on all five micronuclear chromosomes in tetrahymena thermophila. Mol Cell Biol 3(11):1909-19.
Vector TypepUC18
E. coli selectionAmpicillin
Tetrahymena selectionNone
Selection cassette
Expression cassette
Notes2 kb HindIII fragment of Tetrahymena thermophila micronuclear DNA clones into the HindIII site of pBR322.

DNA sequence is repeated in the micronuclear genome, with major HindIII fragment sizes of 2 and 4 kb.
Sequence Filenot available
Plasmid Mapnot available