Tetrahymena Stock Center| Plasmid ID | PID00027 |
| Plasmid Name | pMBR 4C1 |
| Academic Cost | $60 |
| Base Pairs | 9 |
| Source | Kathleen Karrer, Marquette University, U.S.A. |
| Publications | Wuitschick JD, Gershan JA, Lochowicz AJ, Li S, Karrer KM. 2002. A novel family of mobile genetic elements is limited to the germline genome in tetrahymena thermophila. Nucleic Acids Res 30(11):2524-37. |
| Vector Type | pUC18 |
| E. coli selection | Ampicillin |
| Tetrahymena selection | None |
| Selection cassette | |
| Expression cassette | |
| Notes | Library construction: Tetrahymena thermophila strain CU399 micronuclear DNA partially digested with MboI and filled in with dGTP and dATP, ligated to pUC18 digested with SalI and filled in with dTTP and dCTP. See Wuitschick et al., figure 1 for map of Tlr element family GenBank Accession No. AF451860 |
| Sequence File | not available |
| Plasmid Map |
