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Plasmid IDPID00024
Plasmid NamepMNBL (neo3)
Academic Cost$50
Base Pairs6589
SourceMarty Gorovsky, University of Rochester
PublicationsShang Y, Song X, Bowen J, Corstanje R, Gao Y, Gaertig J, Gorovsky MA. 2002. A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in tetrahymena thermophila. Proc Natl Acad Sci U S A 99(6):3734-9.
Vector TypeParomomycin selection cassette–non-codon optimized
E. coli selectionAmpicillin
Tetrahymena selectionParomomycin
Selection cassette~2.5 kb of MTT1 promoter-pm, inducible promoter
Expression cassette
NotesThis is similar to pNeo3 except that a much longer region of the MTT1 promoter is included.
Sequence File pMNBL.docx
Plasmid Map