Tetrahymena Stock Center| Plasmid ID | PID00024 |
| Plasmid Name | pMNBL (neo3) |
| Academic Cost | $60 |
| Base Pairs | 6589 |
| Source | Marty Gorovsky, University of Rochester |
| Publications | Shang Y, Song X, Bowen J, Corstanje R, Gao Y, Gaertig J, Gorovsky MA. 2002. A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in tetrahymena thermophila. Proc Natl Acad Sci U S A 99(6):3734-9. |
| Vector Type | Paromomycin selection cassette–non-codon optimized |
| E. coli selection | Ampicillin |
| Tetrahymena selection | Paromomycin |
| Selection cassette | ~2.5 kb of MTT1 promoter-pm, inducible promoter |
| Expression cassette | |
| Notes | This is similar to pNeo3 except that a much longer region of the MTT1 promoter is included. |
| Sequence File | pMNBL.docx |
| Plasmid Map |
