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Plasmid IDPID00014
Plasmid NamepXS76
Academic Cost$50
Base Pairsnot available
SourceMarty Gorovsky, University of Rochester
Publications
Vector TypeMTT1 locus
E. coli selectionAmpicillin
Tetrahymena selectionParomomycin
Selection cassetteneo2 [HHFI(promoter)-non-codon optimized neo; constitutive promoter
Expression cassetteMTT1(promoter)-HTA.gamma; inducible promoter
NotesgammaHTA can be removed by restriction with BamHI and AscI, and replaced with GOI targeted to MTT1 locus
Sequence File pXS76_minus_insert.docx
Plasmid Map