Plasmid ID | PID00007 |
Plasmid Name | pchxMTTGFP |
Academic Cost | $60 |
Base Pairs | 8211 |
Source | Douglas Chalker, Washington University, St. Louis, MO |
Publications | Pearson CG, Giddings TH,Jr, Winey M. 2009. Basal body components exhibit differential protein dynamics during nascent basal body assembly. Mol Biol Cell 20(3):904-14. |
Vector Type | Targets rpl29 locus |
E. coli selection | Kanamycin |
Tetrahymena selection | Cycloheximide |
Selection cassette | Mutation in rpl29 conferring cycloheximide resistance |
Expression cassette | MTT1(promoter)-GFP; inducible promoter |
Notes | Linearize plasmid with HindIII, integrates into rpl29 locus |
Sequence File | not available |
Plasmid Map |