| Plasmid ID | PID00003 |
| Plasmid Name | pmCherry-pur4 |
| Academic Cost | $60 |
| Base Pairs | 5882 |
| Source | Tokuko Haraguchi, Osaka University, Japan |
| Publications | Iwamoto M, Mori C, Hiraoka Y, Haraguchi T. 2014. Puromycin resistance gene as an effective selection marker for ciliate Tetrahymena. Gene 534(2):249-55. |
| Vector Type | Vector for adding mCherry to 3' end of a gene and selecting with puromycin |
| E. coli selection | Ampicillin |
| Tetrahymena selection | Puromycin |
| Selection cassette | pPur4-opt [MTT1(promoter)-pur-opt; inducible promoter] |
| Expression cassette | Allows 3' addition of mCherry to GOI. Targeting determined by selection of 5' and 3' flanks. |
| Notes | 5' targeting sequence, including promoter and gene can be added into MCS 5' of mCherry sequence. 3' targeting flank can be added in a second MCS 3' of the Pur4-opt cassette (see map). |
| Sequence File | pmCherry-pur4.docx |
| Plasmid Map |
Tetrahymena Stock Center