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Plasmid IDPID00003
Plasmid NamepmCherry-pur4
Academic Cost$50
Base Pairs5882
SourceTokuko Haraguchi, Osaka University, Japan
PublicationsIwamoto M, Mori C, Hiraoka Y, Haraguchi T. 2014. Puromycin resistance gene as an effective selection marker for ciliate Tetrahymena. Gene 534(2):249-55.
Vector TypeVector for adding mCherry to 3' end of a gene and selecting with puromycin
E. coli selectionAmpicillin
Tetrahymena selectionPuromycin
Selection cassettepPur4-opt [MTT1(promoter)-pur-opt; inducible promoter]
Expression cassetteAllows 3' addition of mCherry to GOI. Targeting determined by selection of 5' and 3' flanks.
Notes5' targeting sequence, including promoter and gene can be added into MCS 5' of mCherry sequence. 3' targeting flank can be added in a second MCS 3' of the Pur4-opt cassette (see map).
Sequence File pmCherry-pur4.docx
Plasmid Map