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Tetrahymena Growth Media

250 μg/ml penicillin G and streptomycin sulfate and 1.25 μg /ml amphotericin B (Fungizone-GIBCO)] can be added to working cultures of any media if desired. Not necessary if sterile conditions are maintained.

Modified Neff media

0.25% proteose peptone
0.25% Difco yeast extract
0.5% glucose
3.33 μM FeCl3

SSP Media

2% proteose peptone
0.1% yeast extract
0.2% glucose
0.003% sequestrene (Fe-EDTA, can be replaced with 33 μM FeCl3)

In all media, growth is limited by iron, which can be supplied by iron salts or chelated iron salts.

PP media

2% proteose peptone
10 μM FeCl3
Removal of glucose and yeast extract is reported not to affect doubling time.