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Stock IDSD01832
Strain NameA5A7-1
SpeciesT. thermophila
Genetic Descriptionatu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E445A,E447A]; ATU1 with E445A,E447A or neo replacing coding of some ATU1 genes, pm-r, cy-s,mp-? )
MAC Knockins/outsATU1 with E445A,E447A and neo replacing coding of some ATU1 genes
MIC Knockins/outsneo replaces ATU1 coding
Gene of InterestATU1 (TTHERM_00558620)
OriginBing Hai
PublicationsHai B and Gorovsky MA. 1997. Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in tetrahymena thermophila. Proc Natl Acad Sci U S A 94(4):1310-5.

Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.
CommentsIt is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pA5A7 derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E445A,E447A

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01832
Requires MTA?No
Academic cost $35