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Stock IDSD01774
Strain NameVI009
AKA
SpeciesT. thermophila
Genetic DescriptionMPR1/mpr1-1; JMJ2/jmj2-1[Δ21-672(::neo4, KanR2)] (MPR1/mpr1-1; JMJ2/jmj2-1[Δ21-672(::neo4, KanR2)]; mp-r, pm-r, ?)
MAC Knockins/outs
MIC Knockins/outsJMJ2 gene partially replaced by neo4-KanR2 cassette.
Gene of InterestJMJ2 (TTHERM_00455500)
OriginGermline biolistic transformation of mating B2086 and CU428 strains using a neo4-KanR2 knockout cassette.
Publications
CommentsThe knockout has been confirmed by 6-methylpurine and paromomycin resistance, but neither micronuclear nor macronuclear allele homozygosity has been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena.

Submitted by Robert Coyne, J. Craig Venter Institute
Research Resource IdentifierTSC_SD01774
Requires MTA?No
Academic cost $35