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Stock IDSD01758
Strain NameVPS13A::NEO
AKATtVPS13AA4PA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1; VPS13A/VPS13A (MPR1; vps13a-1::neo4; pm-r, mp-s, VII, VII)
MAC Knockins/outsknockout of VPS13A
MIC Knockins/outs
Gene of InterestVacuolar Protein Sorting 13A gene
OriginStrain CU428.2 with complete macronuclear replacement of VPS13A gene with allele containing a NEO4 gene insertion and deletion of parts of intron 2 and exon 3.
PublicationsSamaranayake H.S., Cowan A.E., Klobutcher L.A. 2011. Vacuolar protein sorting protein 13A, TtVPS13A, localizes to the tetrahymena thermophila phagosome membrane and is required for efficient phagocytosis. Eukaryotic Cell 10(9):1207-18.

Samaranayake HS. 2010. Assessing the role of tetrahymena thermophila vacuolar protein sorting 13A (TtVPS13A) in phagocytosis. University of Connecticut (PhD thesis).
CommentsDeposited line is phenotypic assorted subclone with no detectable copies of wild type VPS13A gene. Gene has also been referred to as TtVPS13a. Stock has been maintained in SSP medium + 0.1 ug/ml CdCl2 + 6 mg/ml paromomycin.

Submitted by Lawrence Klobutcher, University of Connecticut Health Center
Research Resource IdentifierTSC_SD01758
Requires MTA?No
Academic cost $35