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Stock IDSD01743
Strain NameVI008
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (MPR1; UPF3/upf3-1[Δ11-159(::neo4, KanR2)]; pm-r, VII)
MAC Knockins/outsUPF3 gene partially replaced by neo4-KanR2 cassette.
MIC Knockins/outsPAR1 gene partially replaced by neo4-KanR2 cassette.
Gene of InterestUPF3 (TTHERM_00046240)
OriginSomatic biolistic transformation of CU428 strains using a neo4-KanR2 knockout cassette.
Publications
CommentsThe knockout also introduces a frameshift mutation. The knockout has been confirmed by paromomycin resistance, but macronuclear allele homozygosity has not been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena.

Submitted by Robert Coyne, J. Craig Venter Institute
Research Resource IdentifierTSC_SD01743
Requires MTA?No
Academic cost $35