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Stock IDSD01738
Strain NameVI001
AKA
SpeciesT. thermophila
Genetic DescriptionMPR1/mpr1-1; PAR1/par1-1[Δ17-2587(::neo4, KanR2)] (MPR1/mpr1-1; PAR1/par1-1[Δ17-2587(::neo4, KanR2)]; mp-r, pm-r, ?)
MAC Knockins/outs
MIC Knockins/outsPAR1 gene partially replaced by neo4-KanR2 cassette.
Gene of InterestPAR1 (TTHERM_00502600)
OriginGermline biolistic transformation of mating B2086 and CU428 strains using a neo4-KanR2 knockout cassette.
Publications
CommentsThe knockout also introduces a frameshift mutation. The knockout has been confirmed by 6-methylpurine and paromomycin resistance, but neither micronuclear nor macronuclear allele homozygosity has been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena.

Submitted by Robert Coyne, J. Craig Venter Institute
Research Resource IdentifierTSC_SD01738
Requires MTA?No
Academic cost $35