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Stock IDSD01631
Strain NameII8G-IA(b)
AKAphg-
SpeciesT. thermophila
Genetic Descriptionpresumed phg-/phg- (presumed phg-; phg-, ?)
MAC Knockins/outs
MIC Knockins/outs
Gene of Interest
Origin"Derived from CU399" (Tiedtke et al. 1988). "Preliminary genetic data show that the phg- character is probably caused by a recessive single-gene mutation, that chromosomally maps on chromosome 3" (Hunseler & Tiedtke, 1986. Society of Protozoologists Abstract #124). Obtained from Arno Tiedtke, Feb. 1989.
PublicationsTiedtke A, Hunseler P, Rasmussen L. 1988. Growth requirements of a new food-vacuole-less mutant of tetrahymena. Eur J Protistol 23:350-3.

Kaczanowska J, Kaczanowski S, Kiersnowska M, Fabczak H, Tulodziecka K, Kaczanowski A. 2008. Acquisition of cell polarity during cell cycle and oral replacement in tetrahymena. Int J Dev Biol 52(2-3):249-58.

Frankel J. 2008. What do genic mutations tell us about the structural patterning of a complex single-celled organism? Eukaryot Cell 7(10):1617-39.
CommentsThe Frankel lab could not obtain progeny from crosses using this stock. Its observations are consistent with the reported temperature-sensitive food-vacuoleless phenotype, but not consistent with the reported total loss of oral structures. This stock exhibits multiple unique morphogenetic phenotypes at 37.5o C.: see Frankel (2008: p. 1626, 1632). The Iowa stock is designated II8G-IA to distinguish it from the II8G stock, deposited by L. Rasmussen at the American Type Culture Collection. ATCC.

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01631
Requires MTA?No
Academic cost $35