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Stock IDSD01591
Strain NameIA322
AKAcdaJ-1 VIIa
SpeciesT. thermophila
Genetic DescriptioncdaJ-1/cdaJ-1 (cdaJ-1; cdaJ, VII)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestCDAJ (cell division arrest J)
OrigincdaJ-1/cdaJ-1 lkn-1/lkn-1 double homozygotes emerged as genomic exclusion progeny following an outcross to B2079-X-II of a mutant clone that resulted from nitrosoguanidine mutagenesis in 1981 of IA264 followed by cytogamy. These genes were separated by two successive outcrosses to B2079-X-II followed by genomic-exclusion crosses, which ultimately yielded cdaJ-1/cdaJ-1 homozygotes including IA322.
CommentsTemperature-sensitive fission arrest with a high degree of disorganization of ciliary rows.Not extensively studied.

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01591
Requires MTA?No
Academic cost $35