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Stock IDSD01518
Strain NameIA328
AKAlkn1-2 III
SpeciesT. thermophila
Genetic Descriptionlkn1-2/lkn1-2 (lkn1-2; III)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestLKN1 (low kinety number 1)
OriginGenomic exclusion progeny following an outcross to B2079-X-II of a mutant clone that resulted from ethyl-methane-sulfonate mutagenesis in 1982 of IA264 followed by cytogamy.
PublicationsBriefly mentioned in Frankel J. 2008. What do genic mutations tell us about the structural patterning of a complex single-celled organism? Eukaryot Cell 7(10):1617-39, p. 1622.
CommentsReduced number of ciliary rows, accompanied by some irregularities in ciliary rows; cells tend to be short and squat. Expression is not temperature-sensitive. Allelic to lkn1-1(IA319): similar phenotype, fully penetrant but more weakly expressed. Fully penetrant at 29o C. Detailed phenotypic analysis was carried out on its sister clone, IA329.

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01518
Requires MTA?No
Academic cost $35