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Stock IDSD01516
Strain NameIA280
SpeciesT. thermophila
Genetic DescriptionmpA-1/mpA-1 (mpA-1; VII)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestMPA (membranellar pattern A) Originally published as mp/mp
OriginA mpA-1/mpA-1, con1-1+/con1-1+ progeny clone derived from an A-star cross of the F1 of a previous con1-1/con1-1 mpA-1/mpA-1 (strain D) x wild-type (stain B) cross. The con-1/con-1 mpA-1/mpA-1 clone was selected by Doerder et al. (1975) following mutagenesis of D1868 cells
PublicationsOriginal mp. Described in detail by Kaczanowski:
Kaczanowski A. 1975. A single-gene-dependent abnormality of adoral membranelles in tetrahymena pyriformis, species 1. Genetics 81(4):631-9.

Kaczanowski A. 1976. An analysis of MP gene affected morphogenesis in tetrahymena pyriformis, syngen 1, (species 1) ciliates. J Exp Zool 196(2):215-30.
CommentsAbnormal oral apparatuses arising during oral replacement as a consequence of incomplete joining of two membranellar subfields. Not temperature-sensitive. Kaczanowski's original description was of non-mutagenized clones of the inbred D strain, which was accidentally homozygous for the naturally-occurring mpA-1 allele. IA280 is a derived offshoot, and shows very low expression in nutrient medium. Expression under culture conditions conducive to oral replacement was not tested.

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01516
Requires MTA?No
Academic cost $35