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Stock IDSD01471
Strain NameSB2377H
AKAPrecursor for meiotic segregant panel 2A
SpeciesT. thermophila
Genetic Descriptionchx1-1/chx1-1; mpr1-1/mpr1-1; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; cy-s, ?)
MAC Knockins/outs
MIC Knockins/outs
Gene of Interest
OriginB x C3 F1 progeny were induced to undergo meiosis by crossing them to A*. This clone is a round I heterokaryon strain. To generate the panel member it must be crossed in mass to A* for two rounds. True progeny are selected for by resistance to cycloheximide.
PublicationsLynch TJ, Brickner J, Nakano KJ, Orias E. 1995. Genetic map of randomly amplified DNA polymorphisms closely linked to the mating type locus of tetrahymena thermophila. Genetics 141(4):1315-25.

Brickner JH, Lynch TJ, Zeilinger D, Orias E. 1996. Identification, mapping and linkage analysis of randomly amplified DNA polymorphisms in tetrahymena thermophila. Genetics 143(2):811-21.
CommentsThis strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, mp-r, pm-s, mat3

Submitted by Eduardo Orias, University of California, Santa Barbara
Research Resource IdentifierTSC_SD01471
Requires MTA?No
Academic cost $35