Stock ID | SD01434 |
Strain Name | IA319 |
AKA | lkn1-1 V |
Species | T. thermophila |
Genetic Description | lkn1-1/lkn1-1 (lkn1-1; V) |
MAC Knockins/outs | |
MIC Knockins/outs | |
Gene of Interest | LKN1 (low kinety number 1) |
Origin | cdaJ-1/cdaJ-1 lkn-1/lkn-1 double homozygotes emerged as genomic exclusion progeny following an outcross to B2079-X-II of a mutant clone that resulted from nitrosoguanidine mutagenesis in 1981 of IA264 followed by cytogamy. These genes were separated by an two successive outcrosses to B2079-X-II followed by genomic-exclusion crosses, which yielded lkn1-1/lkn1-1 homozygotes including IA319. |
Publications | Briefly described in Frankel J. 2008. What do genic mutations tell us about the structural patterning of a complex single-celled organism? Eukaryot Cell 7(10):1617-39, (p. 1622 and Fig.4.). |
Comments | A reduced number of ciliary rows combined with a reduced fidelity in transmission of pre-existing row numbers, probably owing to interruptions and irregularities in the rows. The phenotype is unconditional and penetrance is 100%. Cell mutiplication is unaffected. Submitted by Joseph Frankel, University of Iowa |
Research Resource Identifier | TSC_SD01434 |
Requires MTA? | No |
Academic cost | $45 |