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Stock IDSD01434
Strain NameIA319
AKAlkn1-1 V
SpeciesT. thermophila
Genetic Descriptionlkn1-1/lkn1-1 (lkn1-1; V)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestLKN1 (low kinety number 1)
OrigincdaJ-1/cdaJ-1 lkn-1/lkn-1 double homozygotes emerged as genomic exclusion progeny following an outcross to B2079-X-II of a mutant clone that resulted from nitrosoguanidine mutagenesis in 1981 of IA264 followed by cytogamy. These genes were separated by an two successive outcrosses to B2079-X-II followed by genomic-exclusion crosses, which yielded lkn1-1/lkn1-1 homozygotes including IA319.
PublicationsBriefly described in Frankel J. 2008. What do genic mutations tell us about the structural patterning of a complex single-celled organism? Eukaryot Cell 7(10):1617-39, (p. 1622 and Fig.4.).
CommentsA reduced number of ciliary rows combined with a reduced fidelity in transmission of pre-existing row numbers, probably owing to interruptions and irregularities in the rows.

The phenotype is unconditional and penetrance is 100%. Cell mutiplication is unaffected.

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01434
Requires MTA?No
Academic cost $35