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Stock IDSD01428
Strain NameIA223
SpeciesT. thermophila
Genetic DescriptionpsmA-1/psmA-1 (psmA-1; IV)
MAC Knockins/outs
MIC Knockins/outs
Gene of InterestPSMA (pseudomacrostome A)
OriginFifth-generation progeny derived from an assortant in turn derived from nitrosoguanidine mutagenesis of B1868 VI x VII in 1974. Crosses intervening between the original assortant and the eventual IA223 homozygote include two outcrosses to B1868 VII and B1975 III followed by crosses between heterozygous progeny, and one genomic exclusion cross.
PublicationsFrankel J, Jenkins LM, Bakowska J, Nelsen EM. 1984b. Mutational analysis of patterning of oral structures in tetrahymena. I. effects of increased size on organization. J Embryol Exp Morphol 82:41-66.

Briefly described in Frankel J. 2008. What do genic mutations tell us about the structural patterning of a complex single-celled organism? Eukaryot Cell 7(10):1617-39, (p. 1626-7 and Fig. 10).
CommentsElongated phenotype with development of large oral-replacement or misplaced cell division oral primordia.

Located on chromosome 5. There were other segregating mutations present, and viability problems. IA223 is reliably 100% penetrant even at 28 degrees, with medium-dependent forms of expression, which is reduced at lower temperatures. This clone probably contains an uncharacterized enhancer, since a sibling homozygote, IA222 (psmA-1/psmA-1 III), has a similar phenotype but with much lower penetrance.

Submitted by Joseph Frankel, University of Iowa
Research Resource IdentifierTSC_SD01428
Requires MTA?No
Academic cost $35