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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02626
Strain Nameh∆∆HTA∆∆HTB-FBB1D3
SpeciesT. thermophila
Genetic Descriptionhtax[∆::neo2]/htax[∆::neo2];hta1[∆::neo2]/hta1[∆::neo2] ; htb1[∆::neo2]/htb1[∆::neo2];htb2[∆::neo2]/htb2[∆::neo2 (mp-r?, ?)
MAC Knockins/outs
MIC Knockins/outsneo2 replaces coding of HTAX,HTA1,HTB1,HTB2
Gene of InterestHTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
PublicationsWang Z, Cui B, Gorovsky MA. 2009. Histone H2B ubiquitylation is not required for histone H3 methylation at lysine 4 in tetrahymena. J Biol Chem 284(50):34870-9.

Wang Z. 2007. Functional studies of histone ubiquitylation in tetrahymena thermophila. University of Rochester.
CommentsHTX1/HTB1 quadruple KO heterokaryons;From mating of h∆∆HTA-F14A and h∆∆HTB-B3D3; made with B*VII. Not mating types II/VI/VII.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02626
Requires MTA?No
Academic cost $45