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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD02623
Strain Namegl KO HHT2/HHF2 #10
SpeciesT. thermophila
Genetic Descriptionhhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2] (mpr1-1; mp-r, II)
MAC Knockins/outs
MIC Knockins/outsNeo2 replaces the HHF2 gene and HHT2 gene and the sequence between them
Gene of InterestHHT2 (TTHERM_00189180) + HHF2 (TTHERM_00189170)
PublicationsLiu Y, Song X, Gorovsky MA, Karrer KM. 2005. Elimination of foreign DNA during somatic differentiation in tetrahymena thermophila shows position effect and is dosage dependent. Eukaryot Cell 4(2):421-31.

Liu Y. 2003. Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of tetrahymena thermophila. University of Rochester.
CommentsThe germline knockout heterokaryon of HHT2/HHF2 and the intergenic region has neo cassette completely replacing coding region. 3 independent lines #9, #10, #11.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02623
Requires MTA?No
Academic cost $45