Skip to main content


Follow @TetrahymenaSC

We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.


Stock IDSD02308
Strain NameGTU11 single cell lines #18 and #6
AKA
SpeciesT. thermophila
Genetic Descriptiongtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, K358A,K359A)]; pm-r, ?)
MAC Knockins/outsGTU1 with mutations K358A,K359A anc c-terminus HA tag into the MTT1 locus, partial replacement.
MIC Knockins/outsNeo2 into GTU1 locus
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.

Shang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44.
CommentspGTUK358K359A-HA rescued GTUKO 5x6 progeny having mutation @ GTU1 K358K359 to alanine in GTU1-1 gene, driven by MTT1 promoter. Needs Cd to induce. Has HA tag at c-terminus.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02308
Requires MTA?No
Academic cost $45