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Stock IDSD02301
Strain NameE5+GFP
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (hho1[3’neo2, T35E, S43E, S45E, T47E, T54E, GFPc]; pm-r, VII)
MAC Knockins/outsChange Threonine 35,47,54 and serine 43,45 to glutamic acid
MIC Knockins/outs
Gene of InterestHHO1 (TTHERM_00823720)
OriginYali Dou
PublicationsDou Y, Mizzen CA, Abrams M, Allis CD, Gorovsky MA. 1999. Phosphorylation of linker histone H1 regulates gene expression in vivo by mimicking H1 removal. Mol Cell 4(4):641-7.

Dou Y and Gorovsky MA. 2000. Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch. Mol Cell 6(2):225-31.

Dou Y, Bowen J, Liu Y, Gorovsky MA. 2002. Phosphorylation and an ATP-dependent process increase the dynamic exchange of H1 in chromatin. J Cell Biol 158(7):1161-70.
CommentsComplete somatic tranformant of CU428 cells with pHHOE5-GFP /neo construct. Phosphorylation sites 1- 5 of H1 changed to glutamic adid, neo cassette in 3’ flanking region for selection.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02301
Requires MTA?No
Academic cost $35