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Stock IDSD02197
Strain NameV5-PP50 M2-H7
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (HA tagged HTAY and V5 tagged PPO1; cy-r, pm-r, VII)
MAC Knockins/outsHA tagged HTAY and PPO1 with n-terminal V5 tag
MIC Knockins/outs
Gene of InterestPPO1 (TTHERM_00865290)
OriginJody Bowen
PublicationsSong X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29.
CommentsHA2-HTAY cells transformed with V5-PP50 with the cycloheximide resistance cassette H4-rpL29-B upstream. Partial replacement . Cells are very sick, give yellow-green auto fluorescence with many vacuoles. Do give the correct ~36 kDa Western band for anti V5.

Grow with 15 ug/ml cy and 80 ug/ml pm.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02197
Requires MTA?No
Academic cost $35