Skip to main content


Follow @TetrahymenaSC

Stock IDSD02194
Strain NameTYAHA@MTT1 locus s.c. line #3
AKA
SpeciesT. thermophila
Genetic Descriptiongtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, T270A, Y272A)]; pm-r, ?)
MAC Knockins/outsGTU1 with mutations T270A, Y272A anc c-terminus HA tag into the MTT1 locus, partial replacement.
MIC Knockins/outsNeo2 into GTU1 locus
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.

Shang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44.
CommentspR285D291 rescued GTUKO 5x6 progeny having mutation @ T270,Y272-AA in GTU1-1 gene, driven by MTT1 promoter. Needs Cd to induce. Has HA tag at c-terminus.
#3 can be crossed with #6.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02194
Requires MTA?No
Academic cost $35