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Stock IDSD02019
Strain NameAB2D9-1
AKA
SpeciesT. thermophila
Genetic Descriptionbtu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-?[E431D,E432D,E433D,E435D,E437D,E438D,E439D,E440D,E442D]; ATU1/atu1-1[α-β hybrid tail, AB2]; pm-r, Original BTU2 is replaced by bsr1, BTU1 coding replaced by neo1, then transformed with BTU1 with (E431-442D) and Chimeric ATU1 gene, AB2 (α-β hybrid ), ?)
MAC Knockins/outsBTU2 replaced by bsr. BTU1 partially replaced by neo1, some replaced by BTU1-D9 with (E431-442D). Some ATU1 replaced by ATU1 with chimeric α-β hybrid tail.
MIC Knockins/outsknockout of btu1 with neo
knockout of btu2 with bsr
Gene of InterestBTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580), ATU1 (TTHERM_00558620)
OriginJianming Duan
PublicationsXia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bre MH, Levilliers N, Gorovsky MA, Gaertig J. 2000. Polyglycylation of tubulin is essential and affects cell motility and division in tetrahymena thermophila. J Cell Biol 149(5):1097-6.

Duan J and Gorovsky MA. 2002. Both carboxy-terminal tails of alpha- and beta-tubulin are essential, but either one will suffice. Curr Biol 12(4):313-6.

Thazhath R, Jerka-Dziadosz M, Duan J, Wloga D, Gorovsky MA, Frankel J, Gaertig J. 2004. Cell context-specific effects of the beta-tubulin glycylation domain on assembly and size of microtubular organelles. Mol Biol Cell 15(9):4136-47.
CommentsProgeny of DB6B x DB2A, co- transformed by constructs BTU1-D9 with (E431-442D) and construct of chimeric ATU1 gene, AB2 (α-β hybrid ) See publications.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD02019
Requires MTA?No
Academic cost $35