Skip to main content

Follow @TetrahymenaSC

We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01851
Strain NameDRH1 s ∆ C7-6G
SpeciesT. thermophila
Genetic Descriptionin CU427, chx1-1/chx1-1 (drh1[∆?::neo3]; DRH1 gene 5’ end of coding replaced with Neo3 ,pm-r up to 700ug/ml pm with Cd., VI)
MAC Knockins/outsNeo3 cassette replaces part of 5’ end of coding
MIC Knockins/outs
Gene of InterestDRH1 (TTHERM_00190830)
OriginJody Bowen
CommentsSomatic KO of DRH1 with neo3 KO2 construct. 5’ end of coding removed. Bad maters so not used
See Jody DRH1 logbook
Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryons do not grow well.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01851
Requires MTA?No
Academic cost $45