Tetrahymena Stock Center| Stock ID | SD01824 |
| Strain Name | p115 V5 CU427-2 |
| AKA | |
| Species | T. thermophila |
| Genetic Description | chx1-1/chx1-1 (giw1[3’neo3,V5c]; p115:: p115-V5-neo3, pm-r V5 tagged GIW1p, VI) |
| MAC Knockins/outs | All GIW1 genes replaced by c-terminal V5 tagged version with neo3 in 3’ flanking region |
| MIC Knockins/outs | |
| Gene of Interest | p115, GIWI (TTHERM_01276320) |
| Origin | Kaz Mochizuki ( KM63) |
| Publications | Noto T, Kurth HM, Kataoka K, Aronica L, DeSouza LV, Siu KW, Pearlman RE, Gorovsky MA, Mochizuki K. 2010. The tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus. Cell 140(5):692-703. |
| Comments | All p115 copy in Mac was replaced with p115-V5-neo3 construct in CU427. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. |
| Research Resource Identifier | TSC_SD01824 |
| Requires MTA? | No |
| Academic cost | $45 |
