Stock ID | SD01771 |
Strain Name | MTT-HA-PP50 #3 |
AKA | |
Species | T. thermophila |
Genetic Description | mpr1-1/mpr1-1 (pm-r, VII) |
MAC Knockins/outs | PPO1 gene with 2 HA tags at n-terminus partially replace MTT1 coding, also neo2 in 5’ flanking region of MTT1. |
MIC Knockins/outs | |
Gene of Interest | PPO1 (TTHERM_00865290 and TTHERM_00865300) |
Origin | Jody Bowen |
Publications | Song X, Bowen J, Miao W, Liu Y, Gorovsky MA. 2012. The nonhistone, N-terminal tail of an essential, chimeric H2A variant regulates mitotic H3-S10 dephosphorylation. Genes Dev 26(6):615-29. |
Comments | Used Xiayuan Song’s pXS 75 construct: neo 2 in 5 flank of MTT1 gene. 2 HA tags right after ATG. then PP50 coding sequence. Need 100ug/ml pm in medium to keep the tagged gene present. From the Gorovsky lab, University of Rochester. |
Research Resource Identifier | TSC_SD01771 |
Requires MTA? | No |
Academic cost | $45 |