Tetrahymena Stock Center| Stock ID | SD01740 |
| Strain Name | VI005 |
| AKA | |
| Species | T. thermophila |
| Genetic Description | (LSD2/lsd2-1[Δ1-302(::neo4, KanR2)]; pm-r, II) |
| MAC Knockins/outs | LSD2 gene partially replaced by neo4-KanR2 cassette. |
| MIC Knockins/outs | PAR1 gene partially replaced by neo4-KanR2 cassette. |
| Gene of Interest | LSD2 (TTHERM_00600670) |
| Origin | Somatic biolistic transformation of B2086 strains using a neo4-KanR2 knockout cassette. |
| Publications | |
| Comments | The knockout also deletes 838bp of the 5' flanking region and introduces a frameshift mutation. The knockout has been confirmed by paromomycin resistance, but macronuclear allele homozygosity has not been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena. Submitted by Robert Coyne, J. Craig Venter Institute |
| Research Resource Identifier | TSC_SD01740 |
| Requires MTA? | No |
| Academic cost | $45 |
