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We are now shipping live cells, plasmids, genomic DNA, and media packets, although shipping may take somewhat longer than normal. Contact us with any questions.

Stock IDSD01415
Strain NameTTMG A6
SpeciesT. thermophila
Genetic Descriptiongtu1[∆::neo2]/gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆::GTU1]; pm-r, ?)
MAC Knockins/outsGTU1 into MTT1 locus, partial replacement
MIC Knockins/outsGTU1 replaced by neo2
Gene of InterestGTU1 (TTHERM_00079520)
OriginYuhua Shang
PublicationsShang Y, Tsao CC, Gorovsky MA. 2005. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J Cell Biol 171(6):1035-44.

Shang Y, Li B, Gorovsky MA. 2002. Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. J Cell Biol 158(7):1195-206.
CommentsIn mac at GTU locus, GTU coding region is replaced by neo cassette. At metallothionein locus, two alleles a) MTT coding region, b) GTU coding region . Use the pTTMG construct (GTU coding driven by MTT promoter) to rescue GTU KO heterokaryon, is cadmium dependent. Requires 0.25 to 1 ug/ml Cd to grow.

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01415
Requires MTA?No
Academic cost $45