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We have resumed shipping live cells and will soon ship plasmids and extracted DNA. Transformation services will take longer to resume.


Stock IDSD01374
Strain NameH3.3-Flag-3
AKA
SpeciesT. thermophila
Genetic Descriptionmpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,FLAGn]; pm-r, VII)
MAC Knockins/outsN-terminal His- tag of HHT3, not sure if complete replacement
MIC Knockins/outs
Gene of InterestHTT3 (TTHERM_00016170)
OriginBowen Cui
PublicationsYu L and Gorovsky MA. 1997. Constitutive expression, not a particular primary sequence, is the important feature of the H3 replacement variant hv2 in tetrahymena thermophila. Mol Cell Biol 17(11):6303-10.

Cui B, Liu Y, Gorovsky MA. 2006. Deposition and function of histone H3 variants in tetrahymena thermophila. Mol Cell Biol 26(20):7719-30.
CommentsSomatic replace HHT3 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3

From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Research Resource IdentifierTSC_SD01374
Requires MTA?No
Academic cost $45